Expression of H C Subunits from Clostridium botulinum Types C and D and Their Evaluation as Candidate Vaccine Antigens in Mice

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Abstract
Two proteins representing the heavy-chain subunits of botulinum neurotoxin types C and D were expressed in Escherichia coli , and their vaccine potential was evaluated. Mice were vaccinated with doses ranging from 0.5 to 10 μg and were challenged with 10 to 10 5 50% lethal doses of toxin. For the type C subunit protein, C50, two doses of 2 μg were required for full protection, while, for type D subunit protein, D50, two 1-μg doses were required. A bivalent vaccine consisting of a mixture of these two proteins also provided protection against both botulinum neurotoxin type C and type D challenge. Antibody levels in serum were determined by both enzyme-linked immunosorbent assays and serum neutralization assays

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