Intrastrand Self-complementary Sequences in Bacillus subtilis DNA
- 1 April 1979
- journal article
- research article
- Published by Microbiology Society in Journal of General Microbiology
- Vol. 111 (2) , 353-361
- https://doi.org/10.1099/00221287-111-2-353
Abstract
Intrastrand self-complementary sequences were isolated from the DNA of B. subtilis by hydroxyapatite (HA) chromatography following thermal renaturation of strands separated by chromatography on methylated albumin-kieselguhr (MAK). The intrastrand structures derived from the MAK H strand (HA HII) were biologically active showing transforming activity for a wide variety of markers, as well as hybridization to pulse-labeled and rRNA. Removal of regions of single-strand DNA with S1 nuclease did not significantly alter the biological activity of the self-annelaed molecules. The overall efficiency of transformation and hybridization of the intrastrand self-annealing DNA was low, suggesting that many sequences in the population are neither active in transformation to prototrophy nor transcribed into RNA.This publication has 14 references indexed in Scilit:
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