Targeted RNA fingerprinting: the cloning of differentiallyexpressed cDNA fragments enriched for members of the zinc finger gene family
Open Access
- 1 January 1994
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 22 (13) , 2612-2618
- https://doi.org/10.1093/nar/22.13.2612
Abstract
We have developed and applied a modification of an 'RNA Fingerprinting' protocol previously published by Welsh and McClelland (Nucleic Acids Research 19: 5275 – 5279 1991) such that cDNA fragments which are both differentially-expressed and enriched for members of a specific gene family can readily be identified. cDNA fragments were amplified with an arbitrary primer initially used in the reverse transcription reaction in combination with a member of a primer set which corresponded to a conserved region within a specific gene family. This technique was used to isolate cDNAs encoding a recently described protein kinase as well as an unknown gene that contained a zinc finger. Several other known genes that contained a zinc finger domain and that were differentially-expressed were also isolated.Keywords
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