Cross-reactions among carbonic anhydrases I, II, and III studied by binding tests and with monoclonal antibodies
- 1 August 1982
- journal article
- research article
- Published by Springer Nature in Biochemical Genetics
- Vol. 20 (7-8) , 809-819
- https://doi.org/10.1007/bf00483975
Abstract
Cross-reactions among carbonic anhydrases (CAs) I, II, and III were studied using a variety of antisera: (1) a rabbit antiserum to bovine CA III, (2) mouse antisera to human CA I, CA II, and CA III; and (3) five monoclonal antibodies prepared by the hybridoma technique using splenocytes from a mouse immunized with human CAs I and II and bovine CA III. Cross-reactions between CAs were readily found by binding assays using these antisera. Human CA I, but not human CA II, inhibited the reaction of the rabbit anti-CA III with its homologous antigen. Mouse antisera to CA I or CA II bound the homologous I or II with nearly as great efficiency as the autologous isozyme and sometimes weakly bound CA III. Mouse antisera to CA III frequently bound CA I or II. These cross-reactions were confirmed by the first use of hybridoma-prepared, monoclonal antibodies to CAs. The mouse monoclonal antibodies to CA isozymes varied in the amount of cross-reactivity among I, II, and III: at one extreme, one monoclonal was highly specific for the autologous CA III; at the other extreme, one monoclonal weakly reacted with some examples of CAs I, II, and III.This publication has 29 references indexed in Scilit:
- Cross-reactivity between Thy-1 and a component of intermediate filaments demonstrated using a monoclonal antibodyNature, 1981
- Testosterone-induced, sulfonamide-resistant carbonic anhydrase isozyme of rat liver is indistinguishable from skeletal muscle carbonic anhydrase IIIFEBS Letters, 1981
- A monoclonal antibody that detects a V kappa-TEPC15 idiotypic determinant cross-reactive with a Thy-1 determinant.The Journal of Experimental Medicine, 1981
- Anti-phosphocholine hybridoma antibodies. I. Direct evidence for three distinct families of antibodies in the murine responseThe Journal of Experimental Medicine, 1981
- Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphenylglycolurilBiochemical and Biophysical Research Communications, 1978
- Somatic cell hybridization of mouse myeloma cellsCell, 1976
- The role of tolerance in the lack of cross-reactivity between homologous (structurally related) proteins: Cross-reactions between α-lactalbumins and between lysozymes recognized by antibodies from intolerant rabbitsImmunochemistry, 1973
- THE MECHANISM OF ANTIGENIC STIMULATION OF PRIMARY AND SECONDARY CLONAL PRECURSOR CELLSThe Journal of Experimental Medicine, 1972
- GENETIC AND PHYLOGENETIC VARIATION IN THE DIFFERENT MOLECULAR FORMS OF MAMMALIAN ERYTHROCYTE CARBONIC ANHYDRASES*Annals of the New York Academy of Sciences, 1968
- Selection of Hybrids from Matings of Fibroblasts in vitro and Their Presumed RecombinantsScience, 1964