Lipophilic aldehydes and related carbonyl compounds in rat and human urine
- 1 May 1999
- Vol. 34 (5) , 489-496
- https://doi.org/10.1007/s11745-999-0389-1
Abstract
Rat and human urine samples were analyzed for lipophilic aldehydes and other carbonyl products of lipid peroxidation. The following compounds were identified as their 2,4-dinitrophenyl hydrazones by cochromatography with pure standards using three solvent systems: butanal, butan-2-one, pentan-2-one, hex-2-enal, hexanal, hepta-2,4-dienal, hept-2-enal, octanal, non-2-enal, deca-2,4-dienal, 4-hydroxyhex-2-enal, and 4-hydroxynon-2-enal. In general, fasted rats excreted less of these compounds than fed rats, indicating they were partially of dietary origin or that the endogenous compounds were excreted in a form not susceptible to hydrazone formation. The compounds excreted in human urine were similar to those excreted in rat urine but were present in lower concentrations. Identification of the conjugated forms fo the lipophilic aldehydes and related carbonyl compounds excreted in urine may be a source of information about their reactions in vivo.Keywords
This publication has 43 references indexed in Scilit:
- Inhibition of glycolytic enzymes by endogenous aldehydes: a possible relation to diabetic neuropathiesBiochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 1994
- α-Hydroxyaldehydes, products of lipid peroxidationBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1994
- Production of Aliphatic Aldehydes on Peroxidation of Various Types of Lipids.CHEMICAL & PHARMACEUTICAL BULLETIN, 1991
- Evidence for 4-hydroxyalkenals in rat renal cortex peroxidized by N2-methyl-9-hydroxyellipticinium acetate or celiptium®Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1990
- 4-Hydroxyhexenal: A lipid peroxidation product derived from oxidized docosahexaenoic acidBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1990
- Studies on the formation of aliphatic aldehydes in the plasma and liver of vitamin E-deficient rats.CHEMICAL & PHARMACEUTICAL BULLETIN, 1990
- Lethal effects of a linoleic acid hydroperoxide and its autoxidation products, unsaturated aliphatic aldehydes, on human diploid fibroblastsChemico-Biological Interactions, 1987
- Detection of 4-hydroxynonenal and other lipid peroxidation products in the liver of bromobenzene-poisoned miceBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1986
- Fluorometric liquid chromatographic determination of aliphatic aldehydes arising from lipid peroxides.CHEMICAL & PHARMACEUTICAL BULLETIN, 1986
- Identification of 4-hydroxynonenal as a cytotoxic product originating from the peroxidation of liver microsomal lipidsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1980