Cloning of the Aconitase Gene (acn) of Escherichia coli K12
- 1 June 1986
- journal article
- research article
- Published by Microbiology Society in Microbiology
- Vol. 132 (6) , 1763-1766
- https://doi.org/10.1099/00221287-132-6-1763
Abstract
Lambda phages containing the aconitase gene(acn) of Escherichia coli K12 have been isolated by hybridization with an M13 probe containing part of the aconitase gene (citB) of Bacillus subtilis. Aconitase specific activities are amplified 5- to 18-fold in thermally induced .lambda.acn lysogens and threefold in a strain transformed with a plasmid derivative (pGS181).Keywords
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