Instability during storage of phosphogluco‐isomerase isoenzymes from ryegrasses (Lolium spp.)

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Abstract
The stability during storage of phosphogluco‐isomerase (D‐glucose‐6‐phosphate ketol isomerase EC 5.3.1.9) isoenzymes coded for at the PGI/2 locus has been examined. Extracts were prepared from leaves of several diploid and tetraploid Italian (Lolium multiflorum Lam.) and perennial (Lolium perenne. L.) ryegrass cultivars, as well as from interspecific hybrids. It was clearly demonstrated that extracts from plants homozygous for a specific PGI/2 allele could quickly generate new band forms upon storage. The novel forms were not due to aggregation or disintegration of the original enzyme molecule, and some of the generated bands electrophoresed to gel positions characteristic of other alleles of the same locus. An assessment was also made of the effects of a range of compounds added to the storage buffer. The most likely explanation was that the observed changes were due to the action of proteases, and the implications, especially for those using isoenzymes as genetic markers, are discussed.