Agrobacterium ? and microprojectile ? mediated viral DNA delivery into barley microspore-derived cultures
- 1 April 1990
- journal article
- research article
- Published by Springer Nature in Plant Cell Reports
- Vol. 8 (11) , 680-683
- https://doi.org/10.1007/bf00269992
Abstract
Anther cultures of barley (Hordeum vulgare L. var. “Igri”) were used as targets for Agrobacterium-mediated DNA transfer and direct DNA uptake by particle bombardment. A wheat dwarf virus construct which can replicate to a high copy number in cereal cells provided a sensitive marker for successful DNA delivery. Although DNA delivery was achieved using both procedures, particle bombardment gave more reproducible and higher levels of infection. The ability to deliver DNA into cereal cells which have a high regeneration capacity may provide a route for stable transformation.Keywords
This publication has 29 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- Agroinfection of wheat: Inoculation of in vitro grown seedlings and embryosPlant Science, 1989
- The biolistic processTrends in Biotechnology, 1988
- Genetically Transformed Maize Plants from ProtoplastsScience, 1988
- T-DNA integration and expression in a monocot crop plant after induction of AgrobacteriumNature, 1987
- Transgenic rye plants obtained by injecting DNA into young floral tillersNature, 1987
- Agrobacterium-mediated delivery of infectious maize streak virus into maize plantsNature, 1987
- Expression of Ti plasmid genes in monocotyledonous plants infected with Agrobacterium tumefaciensNature, 1984
- Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.Proceedings of the National Academy of Sciences, 1980
- Transfer of the Agrobacterium tumefaciens TI Plasmid to Avirulent Agrobacteria and to Rhizobium ex plantaJournal of General Microbiology, 1977