Potentiation of Forskolin-Induced Increase of cAMP by Diamide and N-Ethylmaleimide in Rat Pancreatic Islets

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Abstract
In isolated rat pancreatic islets, the effect of diamide and N-ethylmaleimide (NEM) on forskolin- as well as on glucagon-induced elevation of cAMP was studied. Forskolin and glucagon increased cAMP levels in batch-incubated islets. Diamide and NEM further augmented forskolin-induced increase of cAMP levels, whereas glucagon-stimulated elevation of cAMP was not affected. From our data it is likely that under the conditions of the present study, the thiols related to the N5-protein and the catalytic unit are insensitive to oxidation and alkylation. The potential of forskolin-induced cAMP production in intact islet cells by diamide and NEM may be due to inactivation of the thiols related to the N1-protein.