Isolation & characterization of malformin

Abstract
Methods are described for isolation of malformin from culture filtrates of Aspergillus niger. Malformin was found to be a neutral peptide containing 4 amino acids, valine, leucine, isoleucine, and 1/2 cystine. The molar ratio obtained for these amino acids supported a 1:1:1:2 ratio, respectively. It was suggested that malformin is a cyclic peptide. The molecular formula, C23H39N5O5S2, was proposed. The infrared spectrum showed strong adsorption bands at 3.01, 6.06, and 6.54 [mu]. A 2nd malformin was found which contained allo-isoleucine.