Positive and negative regulatory elements are involved in transcriptional control of the rat glucokinase gene in the insulin producing cell line HIT M2.2.2

Abstract
Nested deletion mutants of the 5' flanking region of the β-cell transcription unit of the rat glucokinase gene (rßGK) were fused to the CAT-reporter gene. Transient expression studies in HIT M2.2.2 and BHK21 cells revealed a distal (upstream of −359) and a proximal promoter region (between −278/−49) harbouring positive and negative regulatory elements. DNaseI footprinting revealed three protected areas between nucleotides −190 and −60. DNA-elements playing a crucial role in transcriptional control of the insulin genes (IEB- and CT-motifs) have been detected within the proximal promoter region and contribute to β-cell specific gene regulation. 3' deletion analysis revealed that DNA-elements located downstream from transcription initiation sites (up to +123) contribute to transcriptional regulation.