The Inhibitory Mode of Aphidicolin on DNA Synthesis in NaCl-Treated HeLa Cell Nuclei1
- 1 April 1982
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 91 (6) , 2091-2094
- https://doi.org/10.1093/oxfordjournals.jbchem.a133903
Abstract
Isolated HeLa cell nuclei were treated with NaCl at various concentrations and inhibition by aphidicolin of DNA synthesis in the treated nuclei was studied. The inhibition was either noncompetitive or of the mixed type with respect to each dNTP when the nuclei were treated with NaCl at concentrations lower than 0.08 m. However, aphidicolin was a competitive inhibitor with respect to dCTP and a non-competitive or mixed type inhibitor with respect to the other 3 dNTPs when they were treated with NaCl at concentrations higher than 0.1 m. These results suggest the presence of nuclear factors) responsible for the changes in the inhibitory mode of aphidicolin on endogenous nuclear DNA synthesis.This publication has 6 references indexed in Scilit:
- Differential effects of aphidicolin on replicative DNA synthesis and unscheduled DNA synthesis in permeable mouse sarcoma cellsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1980
- The mode of action of aphidicolin on DNA synthesis in isolated nucleiBiochemical and Biophysical Research Communications, 1980
- The Mode of Inhibitory Action by Aphidicolin on Eukaryotic DNA Polymerase αEuropean Journal of Biochemistry, 1979
- Changes in the level of poly ADP-ribosylation during a cell cycleExperimental Cell Research, 1978
- Initiation of HeLa cell DNA synthesis in a subnuclear system.Proceedings of the National Academy of Sciences, 1978
- Dissociation and reconstitution of the DNA replicase system of HeLa cell nucleiBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1976