Specific Programmed Multiple Development–Thin Layer Chromatography of Furazolidone in Chicken, Turkey, Swine, and Bovine Tissues: Collaborative Study

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Abstract
A specific method for assay of furazolidone at 2 ppb has been developed using programmed multiple development-thin layer chromatography (PMD-TLC) and the conversion of the drug to a fluorescent species which is quantitated by fluorodensitometry on the TLC plate. The method requires only 5 g tissue, specifies an automatic spotter for PMD-TLC, and is capable of measuring <1 ng of drug when the fluorodensitometer is coupled with a computing integrator. This procedure requires an average of 2 man-hours per sample and can be completed in one day. Six laboratories collaboratively studied the method for assay of furazolidone at the 2, 3, and 6 ppb levels in chicken, turkey, swine, and bovine tissues. Tissues were fortified by each laboratory and then processed through extraction, solvent partition, programmed multiple development-thin layer chromatography, and fluorodensitometry. Results showed satisfactory recoveries and accuracy. A statistical comparison of the results demonstrated that 5 of the 6 laboratories obtained similar results. The higher responses from the sixth laboratory appeared to be due to an exceptional vacuum system and the use of silated flasks. The mean drug recovery response for the tissues spiked at 2 ppb from the 5 laboratories was 2.2324 ng ± 20.2% (SD), which at the 99.7% confidence level gives no overlap with control tissue data. The results can be expected to be repeatable within and among laboratories.

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