Time-dependent inhibition of insulin release: glucose-arginine interactions in the perfused rat pancreas
- 1 February 1984
- journal article
- research article
- Published by Springer Nature in Diabetologia
- Vol. 26 (2) , 146-149
- https://doi.org/10.1007/bf00281123
Abstract
The isolated perfused rat pancreas was stimulated sequentially with arginine or glucose to analyze the time-dependent modulation of insulin release. A 10-min perfusion with arginine (5.0 mmol/l) induced 75% inhibition of the insulin response to repeated arginine stimulation 10 min later. When glucose (8.3 mmol/l) was given as two pulses, inhibition of the second insulin response was less pronounced. The inhibitory effect generated by arginine also suppressed the insulin response to glucose (27.7 mmol/l), and this inhibitory effect persisted for over 80 min. Stimulation for 30 min with glucose (27.7 mmol/l) strongly potentiated the insulin responses to a pair of arginine stimuli given 20 min later. However, despite augmented secretion rates, the insulin response to the second arginine pulse was still inhibited by 75%. When insulin secretion was strongly amplified by two 10 min pulses of the synergistic mixture of arginine (5.0 mmol/l) and glucose (8.3 mmol/l), there was no inhibition of the second insulin response. If glucose (8.3 mmol/l) was present during the first arginine stimulation only, the response to the second arginine pulse was inhibited as in control experiments. However, when glucose was added to the second arginine pulse only, the inhibition generated by the first arginine pulse did not express itself, insulin release remaining similar to control. We conclude that: (1) short stimulations of the pancreas by arginine or glucose generate long-lasting inhibition of the insulin response to subsequent stimulations; (2) synergistic amplification of the insulin response by addition of glucose to arginine obliterates the inhibition; (3) glucose does not suppress the induction of inhibition, it blocks the expression of the inhibitory signal on insulin secretion; (4) these in vitro findings are in keeping with observations in normal and hyperglycaemic man.This publication has 11 references indexed in Scilit:
- Time-dependent inhibition of insulin release: suppression of the arginine effect by hyperglycaemiaDiabetologia, 1984
- Comparison of storage- and signal-limited models of pancreatic insulin secretionAmerican Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 1980
- Evidence for priming and inhibitory effects of glucose on insulin secretion from isolated islets of langerhansDiabetologia, 1980
- POTENTIATION AND INHIBITION OF INSULIN RELEASE IN MAN FOLLOWING PRIMING WITH GLUCOSE AND WITH ARGININE – EFFECT OF SOMATOSTATINActa Endocrinologica, 1979
- Immediate and Time-Dependent Effects of Glucose on Insulin Release from Rat Pancreatic TissueJournal of Clinical Investigation, 1978
- Potentiation of insulin release by glucose in man. II. Role of the insulin response, and enhancement of stimuli other than glucose.1975
- Feed-back inhibition of insulin secretion in subjects with high and low insulin response to glucose.1975
- A Mathematical Model for the Glucose Induced Insulin Release in ManEuropean Journal of Clinical Investigation, 1974
- A threshold distribution hypothesis for packet storage of insulin and its mathematical modelingJournal of Clinical Investigation, 1972
- Dynamics of Insulin Secretion by the Perfused Rat PancreasEndocrinology, 1968