Laterobasal membranes from intestinal epithelial cells: Isolation free of intracellular membrane contaminants
- 1 October 1987
- journal article
- research article
- Published by Springer Nature in The Journal of Membrane Biology
- Vol. 98 (3) , 197-205
- https://doi.org/10.1007/bf01871183
Abstract
Summary A simplified method for isolating highly purified laterobasal membranes (LBM) from enterocytes is based on treatment of membranes with 8mm CaCl2 concentration in order to aggregate intracellular membrane contaminants. The resultant LBM showed an average 15-fold enrichment and constituted 8% of the original K-stimulated phosphatase in the initial crude homogenate. It showed typical LBM migration on counter-current distribution (CCD) and was essentially free of contamination with endoplasmic reticulum and Golgi membranes. This method is highly efficient and yields sufficient purified LBM to allow comprehensive analysis of enterocyte membrane events.Keywords
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