Expression of ACC oxidase antisense gene inhibits ripening of cantaloupe melon fruits
- 1 July 1996
- journal article
- Published by Springer Nature in Nature Biotechnology
- Vol. 14 (7) , 862-866
- https://doi.org/10.1038/nbt0796-862
Abstract
The plant hormone ethylene plays a major role in the ripening of climacteric fruit. We have generated transgenic cantaloupe Charentais melons expressing an antisense ACC oxidase gene; ACC oxidase catalyzes the last step of ethylene biosynthesis. Ethylene production of transgenic fruit was < 1% of control untransformed fruit, and the ripening process was blocked both on and off the vine. The antisense phenotype could be reversed by exogenous ethylene treatment. Analysis of antisense ACC oxidase melons indicated that the ripening process includes ethylene-dependent and ethylene-independent pathways. Because the transgenic line we generated displays extended storage life and improved quality, it has a promising potential for commercial development.Keywords
This publication has 31 references indexed in Scilit:
- Structure and expression of three genes encoding ACC oxidase homologs from melon (Molecular Genetics and Genomics, 1996
- Internal carbon dioxide and ethylene levels in ripening tomato fruit attached to or detached from the plantPhysiologia Plantarum, 1993
- Ethylene BiosynthesisAnnual Review of Plant Biology, 1993
- Reduced ethylene synthesis in EFE antisense tomatoes has differential effects on fruit ripening processesPostharvest Biology and Technology, 1993
- Altered fruit ripening and leaf senescence in tomatoes expressing an antisense ethylene‐forming enzyme transgeneThe Plant Journal, 1993
- A maize protein associated with the G-box binding complex has homology to brain regulatory proteins.Plant Cell, 1992
- Enhancement of Shoot Regeneration from Cotyledons of Cucumis melo by AgNO3, an Inhibitor of Ethylene ActionJournal of Plant Physiology, 1992
- Control of ethylene synthesis by expression of a bacterial enzyme in transgenic tomato plants.Plant Cell, 1991
- Reversible Inhibition of Tomato Fruit Senescence by Antisense RNAScience, 1991
- Antisense gene that inhibits synthesis of the hormone ethylene in transgenic plantsNature, 1990