Estrogen Receptors in the Human Uterus: Characterization by Sucrose Gradient Centrifugation*
- 1 August 1972
- journal article
- other
- Published by The Endocrine Society in Endocrinology
- Vol. 91 (2) , 594-597
- https://doi.org/10.1210/endo-91-2-594
Abstract
The cytoplasmic estrogen—receptor of the human uterus was examined by sucrose gradient centrifugation. Peaks were identified at 8–9 S and 3–4 S. The receptor was further characterized by salt dissociation and competition experiments. Dextran—charcoal assay revealed a maximal binding capacity of 7.7 X 10-14 moles/mg cytosol protein, and a dissociation constant of 4.4 X10-9M. The characteristics of the estrogen receptors in the cytosol of the human uterus are similar to those reported in other species. (Endocrinology91: 594,1972)Keywords
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