Kinetic limitations in the overall reaction of mitochondrial oxidative phosphorylation accounting for flux‐dependent changes in the apparent ΔGexP/ΔH+ ratio

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Abstract
Changes in J 0, ΔH+ and ΔG ex P were investigated as a function of load. The flux control coefficients, particularly those of the adenine nucleotide translocator and H+‐ATPase at the maximum rate of oxidative phosphorylation were seen to strongly depend on the phosphate concentration accounting in common for the highest share in flux control. There was no unique relationship observed between J P and ΔH+ in load‐controlled, well coupled systems, but J P was found to depend on ΔH+ at excessive load and increasing proton leakage. All the results presented can be elucidated on the grounds of delocalized chemiosmotic coupling.