The Influence of Elongation‐Factor‐Tu · GTP and Anticodon‐Anticodon Interactions on the Anticodon Loop Conformation of Yeast tRNATyr

Abstract
The interactions of yeast tRNATyr, spin‐labelled at position i6A‐37 next to the anticodon, with EF‐Tu · GTP and with Escherichia coli tRNAVal (which has a complementary anticodon) have been studied. The immobilization of the spin label upon ternary complex formation shows a conformational change of the anticodon region, although this part of tRNATyr is not in direct contact with the protein, as indicated by RNase T1 digestion. Upon anticodon‐anticodon interaction, no conformational change of the anticodon loop of tRNATyr was observed.