Monoclonal antibodies to mitochondrial coupling factor B
- 1 January 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 179 (1) , 143-147
- https://doi.org/10.1016/0014-5793(85)80209-x
Abstract
Two monoclonal antibodies (MAb 1 and IV) have been prepared which showed high and specific reactions towards bovine heart mitochondrial coupling factor B (FB). Both have been identified as sub-type IgG1 of mouse immunoglobulins. MAb I reacts with purified and functionally active fB, alkylated or oxidized forms of FB and even with peptides formed on digestion of FB with trypsin. When used together, MAb I and IV reacted with FB in immunoblots of normal and urea treated samples of mitochondria, submitochondrial particles, ammonia-EDTA extracted particles, and H+-ATPase. Both MAbs inhibited FB-stimulated ATP-dependent reverse electron flow activity when fB was incubated with the antibody either before or after its addition to FB-deficient AE-particles. Reactivity of MAb I towards fB declined upon exposure of fb to guanidine HCl while reactivity of MAb IV remained unaltered.Keywords
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