Transfer RNAs of potato (Solanum tuberosum) mitochondria have different genetic origins

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Abstract
Total transfer RNAs were extracted from highly purified potato mitochondria. From quantitative measurements, the in vivo tRNA concentration in mitochondria was estimated to be in the range of 60 .mu.M. Total potato mitochondrial tRNAs were fractionated by two-dimensional polyacrylamide gel electrophoresis. Thirty one individual tRNAs, which could read all sense codons, were identified by aminoacylation, sequencing or hybridization to specific oligonucleotides. The tRNA population that we have characterized comprises 15 typically mitochondrial, 5 ''chloroplast-like'' and 11 nuclear-encoded species. One tRNAAla, 2 tRNAsArg, 1 tRNAIle, 5 tRNAsLeu and 2 tRNAsThr were shown to be coded for by nuclear DNA. A second, mitochondrial-encoded, tRNAIIe was also found. Five ''chloroplast-like'' tRNAs, tRNATrp, tRNAAsn, tRNAHis, tRNASer(GGA) and tRNAMetm, presumably transcribed from promiscuous chloroplast DNA sequences inserted in the mitochondrial genome, were identified, but, in contrast to wheat (1), potato mitochondria do not seem to contain ''chloroplast-like'' tRNACys and tRNAPhe. The two identified tRNAsVal, as well as the tRNAGly, were found to be coded for by the mitochondrial genome, which again contrasts with the situation in wheat, where the mitochondrial genome apparently contains no tRNAVal or tRNAGly gene (2).

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