Construction of a vector pRSVcatamb38for the rapid and sensitive assay of amber suppression in human and other mammalian cells
Open Access
- 1 January 1985
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 13 (4) , 1317-1326
- https://doi.org/10.1093/nar/13.4.1317
Abstract
We describe the generation of an amber mutation in the chloramphenicol acetyltransferase (cat) gene of the mammalian cell transfection vector pRSVcat (Gorman et.al. (1982), Proc.Natl.Acad.Sci. 79 6777–6791). We have demonstrated the in vivo suppression of this amber mutation in monkey and human cells by cotransfection with a synthetic Xenopus suppressor tRNATyr under the control of the late SV40 promoter. The vector, pRSVcatamb38, may be used to quantitate amber suppression in various mammalian cells.Keywords
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