Structure of the dnaA region of Pseudomonas putida: Conservation among three bacteria, Bacillus subtilis, Escherichia coli and P. putida
- 1 February 1989
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 215 (3) , 381-387
- https://doi.org/10.1007/bf00427033
Abstract
Summary We have cloned from Pseudomonas putida a gene homologous to Escherichia coli dnaA, and determined the sequence of the gene and its neighboring region. The dnaA gene and at least three other genes, dnaN, recF and gyrB, were found to be highly homologous to the genes in the dnaA regions of the E. coli and Bacillus subtilis chromosomes. A non-translatable region of some 600 bp immediately upstream of the dnaA gene is also conserved in the three bacteria and contains 3, 12, and 14 DnaA-boxes (TTATCCACA and closely related sequences) in E. coli, P. putida and B. subtilis, respectively. The present results confirm our hypothesis that the dnaA region is the replication origin region of the ancestral bacterium and that the essential feature of the dnaA and DnaA-box combination is conserved in most eubacteria and plays a central role in initiation of chromosomal replication.Keywords
This publication has 20 references indexed in Scilit:
- ATP activates dnaA protein in initiating replication of plasmids bearing the origin of the E. coli chromosomeCell, 1987
- Nalidixic acid-resistant mutations of the gyrB gene of Escherichia coliMolecular Genetics and Genomics, 1986
- Evolutionary change in 5S rRNA secondary structure and a phylogenic tree of 352 5S rRNA speciesBiosystems, 1986
- Sites of dnaA protein-binding in the replication origin of the Escherichia coli K-12 chromosomeJournal of Molecular Biology, 1985
- Physical mapping and nucleotide sequence of the rnpA gene that encodes the protein component of ribonuclease P in Escherichia coliGene, 1985
- The dnaA protein complex with the E. coli chromosomal replication origin (oriC) and other DNA sitesCell, 1984
- Replication origin of the Bacillus subtilis chromosome determined by hybridization of the first-replicating DNA with cloned fragments from the replication origin region of the chromosomeGene, 1984
- Structural analysis of the dnaA and dnaN genes of Escherichia coliGene, 1984
- Effect of novobiocin on initiation of DNA replication in Bacillus subtilisNature, 1979
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977