Experiments on Young Chick Embryos Cultured in Vitro

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Abstract
On young chick embryos of primitive-streak to head-process stages cultured in vitro, a median sagittal cut from the anterior part of the streak to the germ wall gave partial or incomplete doubling of the neural tube, foregut, and notochord, but not of the eye. The epiblast, when cultured separately, gave nervous tissue, mesoderm, and notochord. Removal of Hensen''s node and a variable amount of the node field did not seem to inhibit the posterior differentiation of the neural tube and somites. Notochord was absent. The result depended on the size of the piece removed. When cultured by itself, this piece gave nervous tissue, pulsating heart tissue, and somites. Embryos were also cut transversely.