Propagation of Salivary Gland Virus of the Mouse in Tissue Cultures.

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Abstract
Cytologic changes including large in-tranuclear inclusions were produced in roller tube cultures of mouse embryonic tissue inoculated with salivary gland material from mice infected with the salivary gland virus. The cytologic changes occurred in serial subcultures inoculated with super-natant fluid from preceding cultures. After 3 serial passages of the infective agent in tissue cultures the salivary gland virus disease, as characterized by specific intranuclear inclusions, was reproduced in the salivary glands of mice by intraperitoneal inoculation of the supernatant fluids from the cultures. The infective titers of supernatant fluids withdrawn from cultures at intervals were determined by titrations in mice 3 to 5 weeks of age. In titrations the microscopic demonstration of the char-acteristic intranuclear inclusions in the salivary glands of mice killed 2 weeks following intraperitoneal inoculation was used as the criterion for infection with the virus. The results of the titrations, together with the calculated dilutions of the or-iginal inocula effected during the course of the experiments, demonstrated signiicant increase in the virus during 3 serial passages in tissue cultures.