IMMUNOGENIC AND ANTIGENIC EPITOPES OF IMMUNOGLOBULINS .1. CROSS-REACTIVITY OF MURINE MONOCLONAL-ANTIBODIES TO HUMAN-IGG WITH THE IMMUNOGLOBULINS OF CERTAIN ANIMAL SPECIES
- 1 January 1982
- journal article
- research article
- Vol. 45 (1) , 71-77
Abstract
Antibody-producing hybridoma clones were isolated following immunization of mice with human IgG. Twenty-five monoclonal antibodies (9 anti-C.gamma.3, 14 anti-C.gamma.2, 1 anti-.kappa. and 1 anti-.lambda.) were selected for study of their cross-reactivity with the IgG of 15 mammalian species and chicken Ig. Each antibody exhibited a unique reaction profile suggesting that human IgG expresses a very large repertoire of immunogenic epitopes. Some antibodies showed a very restricted cross-reactivity profile; for others a very wide reactivity profile was observed, including 2 clones producing autoantibodies. Antibodies demonstrating cross-reactivity between human Fc.gamma. and 7S chicken Ig allow its definitive assignment as a homolog of human IgG. Four clones demonstrated specificity for bovine IgG subclass .gamma.1 and .gamma.2 and the degree of reactivity allows their application to qualitative and quantitative assay systems. New perspectives for the characterization of Ig and the standardization of anti-Ig reagents are suggested.This publication has 23 references indexed in Scilit:
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