EFFECT OF MICROTUBULE-ASSOCIATED PROTEINS ON THE INTERACTION OF VINCRISTINE WITH MICROTUBULES AND TUBULIN
- 1 January 1979
- journal article
- research article
- Vol. 39 (5) , 1604-1610
Abstract
The influence of microtubule-associated proteins on the interaction of vincristine [a cancer chemotherapeutic] with [bovine brain] microtubules and tubulin was examined. Microtubules containing associated proteins were prepared in vitro from tubulin preparations which contained the proteins or from pure 6S tubulin and isolated microtubule-associated proteins. The presence of the associated proteins caused microtubules to be converted to stable spiral structures upon reacting with vincristine. When the proteins were absent, spirals were not formed, and the microtubules were completely disassembled by vincristine. At 0.degree. C, 6S tubulin was converted to amorphous aggregates by vincristine, whereas if the associated proteins were present, spirals were formed.This publication has 3 references indexed in Scilit:
- Intracellular localization of the high molecular weight microtubule accessory protein by indirect immunofluorescence.The Journal of cell biology, 1978
- Immunofluorescence Localization of Proteins of High Molecular Weight Along Intracellular MicrotubulesScience, 1977
- ACTION OF VINCA ALKALOIDS VINCRISTINE, VINBLASTINE, AND DESACETYL VINBLASTINE AMIDE ON MICROTUBULES INVITRO1976