Replication of mycoplasmavirus MVL51: Attachment of MVL51 parental DNA to host cell membrane

Abstract
The replication of the single-stranded circular DNA of MLV51 mycoplasmavirus [in Acholeplasma laidlawii] was studied with respect to the roles of free and membrane-associated viral DNA intermediates. Replication involves the formation of parental replicative intermediate (RF) molecules on, at most, 2-3 membrane sites/cell, symmetric RF replication at the membrane, and apparent asymmetric RF replication in the cytoplasm leading to single-stranded progeny chromosomes.