Effect of diethylsulphoxide on growth, survival and ion exchange of Escherichia coli

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Abstract
Aims: To study the effect of diethylsulphoxide (DESO) on Escherichia coli growth, survival and ionic exchange in comparison with dimethylsulphoxide (DMSO). Methods and Results: Bacterial survival was estimated by counting colony‐forming units and by the most probable number (five‐tube) technique; the K+ and H+ transport and H2 formation were determined electrochemically. Diethylsulphoxide at concentrations between 0·01 and 0·5% (w/v) stimulated and above 5% decreased the anaerobic growth rate and survival. 2H+ : K+ exchange and H2 formation were lost at 5% DESO. At 0·05% DESO the kinetic characteristics of H+ : K+ exchange and H2 formation were typical for ΔμH+‐dependent TrkA uncoupled with F0F1 under respiration. Conclusions: Diethylsulphoxide at low concentrations serves as an electron acceptor for an anaerobic respiratory chain stimulating bacterial growth and survival through the modulation of H+ : K+ exchange and H2 formation activity. The effects of DESO were more pronounced than those of DMSO. Significance and Impact of the Study: Diethylsulphoxide determines essential biological and therapeutic properties that make its application preferable.

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