Structural study of rat thyroid transcription factor 1 homeodomain (TTF‐1 HD) by nuclear magnetic resonance
- 28 December 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 336 (3) , 397-402
- https://doi.org/10.1016/0014-5793(93)80845-l
Abstract
The 500 MHz 1H NMR spectrum of a 68-residue peptide, encompassing the rat thyroid transcription factor 1 homeodomain (TTF-1 HD), was fully assigned using standard 2D NMR methodology. The secondary structure elements and their spatial organization were determined and led to a structure very similar to that previously described for other homeodomains and expected also for TTF-1 HD from homology modeling predictions. The three-dimensional arrangement of the three helix fragments of TTF-1 HD preserves the helix-tum-helix motif commonly occurring in many classes of DNA-binding proteinsKeywords
This publication has 33 references indexed in Scilit:
- Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genesPublished by Elsevier ,2004
- Secondary structure of the oct‐3 POU homeodomain as determined by 1H‐15N NMR spectroscopyFEBS Letters, 1993
- TRANSCRIPTION FACTORS: Structural Families and Principles of DNA RecognitionAnnual Review of Biochemistry, 1992
- Dissection of helix capping in T4 lysozyme by structural and thermodynamic analysis of six amino acid substitutions at Thr 59Biochemistry, 1992
- Efficient computation of three-dimensional protein structures in solution from nuclear magnetic resonance data using the program DIANA and the supporting programs CALIBA, HABAS and GLOMSAJournal of Molecular Biology, 1991
- Determination of the three-dimensional structure of theAntennapedia homeodomain fromDrosophila in solution by1H nuclear magnetic resonance spectroscopyJournal of Molecular Biology, 1990
- Analysis of the relationship between side-chain conformation and secondary structure in globular proteinsJournal of Molecular Biology, 1987
- Interactive program for visualization and modelling of proteins, nucleic acids and small moleculesJournal of Molecular Graphics, 1986
- Coherence transfer by isotropic mixing: Application to proton correlation spectroscopyJournal of Magnetic Resonance (1969), 1983
- Fourier transform multiple quantum nuclear magnetic resonanceFaraday Symposia of the Chemical Society, 1978