Flow cytometric characterization and enumeration of Chrysochromulina polylepis during a bloom along the Norwegian coast

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Abstract
Flow cytometry was used to count the concentration of the alga Chrysochromulina polylepis Manton and Parke during a bloom along the Western and Southern Norwegian coasts. Fresh samples could be counted without fixation immediately after sampling. Flow-cytometric enumeration of the alga agreed well with direct counting in the microscope. C. polyepis was identified by its characteristic combination of red fluorescence and scatter signal. These signature coordinates did not change significantly with time, depth or concentration. The flow-cytometric measurements could be preformed even when the ship was running and in normal rough seas in offshore locations, whereas direct counting was impossible under such circumstances.

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