A satisfactory medium and technique for the mass cultivation of Sphaerophorus necrophorus

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Abstract
A modified casitone (MC) medium was used successfully in the cultivation of the pathogenic non-sporing anaerobe, Sphaerophorus necrophorus. The medium is suitable for various physiological, biochemical, and immunological assays of the test organism and obviates the need for frequent passage to susceptible animal hosts. When cultured under a 5% CO2–95% N2 atmosphere at 37 °C, the logarithmic phase of growth occurred at 18–24 h postinoculation. A detailed description of an inexpensive apparatus and the technique for the large-scale production of S. necrophorus is included.

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