Nature of primary product(s) of D‐glucose 6‐phosphate dehydrogenase reaction 13C and 31P NMR study

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Abstract
Glucose 6‐phosphate dehydrogenase catalyzes the oxidation of glucose 6‐phosphate, resulting in the formation of 6‐phosphogluconolactone, As this compound is unstable, it has not been characterized directly. NMR provides a way to directly monitor all components of a reaction and study their structure. Here we report some results on the glucose 6‐phosphate dehydrogenase reaction using 31P and 13C‐NMR. Our results indicate that two different lactones, namely γ (1=4 and δ (1=5) 6‐phosphogluconolactones, are formed as products in this reaction. This is in contrast to an earlier suggestion that glucose 6‐phosphate dehydrogenase produces only the δ‐lactone. On the basis of these results, a new mechanisms for dehydrogenation of the sugar phosphate is proposed.

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