In SituHybridization Histochemistry: A Technique for the Study of Gene Expression in Single Cells
- 1 January 1983
- journal article
- research article
- Published by Mary Ann Liebert Inc in DNA
- Vol. 2 (2) , 157-163
- https://doi.org/10.1089/dna.1983.2.157
Abstract
The patterns of growth hormone and proopiomelanocortin gene expression in the rat pituitary were used to detail the application of the in situ hybridization histochemistry technique in tissue sections. Parameters affecting the efficiency and resolution of hybridization were optimized so that this technique can be used to identify sites of peptide synthesis and to study hormonal regulation of gene expression at the single cell level.This publication has 14 references indexed in Scilit:
- Analysis of Pro-opiomelancortin Gene Structure and FunctionDNA, 1983
- Actin gene expression visualized in chicken muscle tissue culture by using in situ hybridization with a biotinated nucleotide analog.Proceedings of the National Academy of Sciences, 1982
- Immunological method for mapping genes on Drosophila polytene chromosomes.Proceedings of the National Academy of Sciences, 1982
- Accumulation of histone repeat transcripts in the sea urchin egg pronucleusCell, 1981
- Changes in hormone responsiveness of chick oviduct during primary stimulation with estrogenDevelopmental Biology, 1980
- The presence of two α-MSH positive cell groups in rat hypothalamusEuropean Journal of Pharmacology, 1979
- Detection of viral sequences of low reiteration frequency by in situ hybridization.Proceedings of the National Academy of Sciences, 1978
- Nucleotide sequence and amplification in bacteria of structural gene for rat growth hormoneNature, 1977
- Quantitation of globin mRNA levels during erythroid development in the rabbit and discovery of a new β-related species in immature erythroblastsCell, 1977
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977