An alternative protein factor which binds the internal promoter ofXenopus5S ribosomal RNA genes
Open Access
- 1 January 1987
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 15 (21) , 8679-8691
- https://doi.org/10.1093/nar/15.21.8679
Abstract
In small oocytes of Xenopus species, two sets of 5S RNA genes, oocytetype and somatic-type, are fully activated. The 5S RNA transcripts are temporarily stored, half in association with TFIIIA to form a 7S particle, the other half in association with tRNA and two proteins (p48 and p43) to form a 42S particle. It has been established previously that TFIIIA binds to the intenal control region of JS RNA genes and promotes their transcription. Here we show that protein can be translocated from the 42S particles to 5S RNA genes, but only after treatment of the particles with ribonuclease. Nevertheless, once transferred, stable protein-DNA complexes are formed and DNase-protection experiments ahow that binding is specific to the gene promoter, covering exactly the same sequence as TFIIIA. The DNA-binding protein is identified as p48 which, after isolation by ion-exchange chromatography, wil1 bind to 5S RNA genes in the absence of ribonuclease.Keywords
This publication has 27 references indexed in Scilit:
- Gene regulation: Fingers and DNA half-turnsNature, 1986
- A bacteriophage RNA polymerase transcribes through a Xenopus 5S RNA gene transcription complex without disrupting itCell, 1986
- Formation of a rate-limiting intermediate in 5S RNA gene transcriptionCell, 1985
- Immunological identity of proteins that bind stored 5S RNA in Xenopus oocytesExperimental Cell Research, 1984
- The reactivation of developmentally inert 5S genes in somatic nuclei injected into Xenopus oocytesNature, 1981
- Biochemical Research on Oogenesis. Composition of the 42-S Storage Particles of Xenopus laevis OocytesEuropean Journal of Biochemistry, 1980
- Specific interaction of a purified transcription factor with an internal control region of 5S RNA genesCell, 1980
- A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3′ border of the regionCell, 1980
- A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5′ border of the regionCell, 1980
- Biochemical research on oogenesisDevelopmental Biology, 1977