MULTILAYERED FILM ANALYZER - GLUCOSE IN SERUM, PLASMA, CEREBROSPINAL-FLUID, AND URINE - AND UREA NITROGEN IN SERUM AND PLASMA

  • 1 January 1980
    • journal article
    • research article
    • Vol. 26  (1) , 133-137
Abstract
Some basic laboratory performance criteria were studied for the Eastman Kodak glucose and urea nitrogen analyzer. Serum, plasma, urine and cerebrospinal fluid [from humans] were tested. Precision, within-day and between-day, for both analytes was < 2.2% (CV). Both analytes demonstrated linearity, with .apprx. 100% recovery of added substrates. Comparisons with continuous-flow procedures demonstrated good correlation. A variety of sera and plasmas can be used for glucose analysis; oxalate-fluoride-treated plasma is unacceptable for use in analysis for urea nitrogen. No effects were seen on glucose or urea nitrogen analysis from hemolysis, lipemia, icterus, some common drugs, ammonia or abnormally high protein concentrations. Minimum amounts detectable were: glucose (serum) 104 mg/l; glucose (urine) 110 mg/l; glucose (cerebrospinal fluid) 100 mg/l; and urea nitrogen (serum) 21 mg/l. Calibration procedures are discussed for protein-based and aqueous samples.

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