Multiple Molecular Forms of Avian Aldolases. V. Purification and Molecular Properties of Chicken (Gallus domesticus) Liver Aldolase

1 June 1971

journal article
Published by Canadian Science Publishing in Canadian Journal of Biochemistry

Vol. 49 (6) , 647-657
https://doi.org/10.1139/o71-093

Abstract

Aldolase (fructose-1,6-diphosphate D-glyceraldehyde-3-phosphate-lyase, EC 4.1.2.13) was purified from chicken liver. The enzyme was shown to be homogeneous according to the following criteria: purification to a constant specific activity following sequential chromatography on carboxymethyl-Sephadex and Sephadex G-200, electrophoresis on cellulose acetate strips, sedimentation velocity analysis, absence of 10 other glycolytic enzymes, and immunodiffusion in agar.The sedimentation coefficient (s°_20w 8.0), Stokes radius (47 Å), diffusion constant (D°_20w 4.0 × 10⁻⁷ cm²/s), and molecular weight (160 000) are similar to those of rabbit liver aldolase and the muscle and brain enzymes from both chickens and rabbits.

Keywords

This publication has 0 references indexed in Scilit: