Light and electron microscopy of conjugation in the yeast, Schizosaccharomyces octosporus

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Abstract
Conjugation in Schizosaccharomyces octosporus is described through the use of interference contrast microscopy, fluorescence microscopy, and electron microscopy of serial sections. At the light microscope level, mating was frequently observed to occur between cells of common ancestry. Fluorescent staining of the nuclei showed that nuclear migration occurs prior to karyogamy, and following diploidization the nucleus then migrates to the end of the cell. A brightly fluorescent spot was found at the apex of the migration nucleus. At the electron microscope level, the results showed that nuclear movement occurs in the presence of cytoplasmic microtubules that are associated with the spindle pole body, the conjugatory nuclei first fuse at or near the spindle pole bodies, and fusion of the spindle bodies occurs apparently by stacking one onto the other.