Human Recombinant Interleukin-2 Is Mitogenic to Human Lymphocytes

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Abstract
Reported herein are the results of studies demonstrating that purified recombinant human interleukin-2 (hrIL-2) is a potent mitogen for lymphocytes of healthy human donors. The specificity of the hrlL-2-induced response was defined in experiments in which mitogenicity of this T cell growth-promoting lymphokine was completely abrogated by blocking the T cell membrane receptor for IL-2 with the anti-Tac monoclonal antibody. Depletion of adherent mononuclear leukocytes markedly reduced lymphocyte reactivity to hrIL-2, but the response could be fully recovered by the addition of interleukin-1 (IL-1). Increased proliferative responses were observed using a combination of hrIL-2 and a monoclonal antibody OKT3 that defines a T cell membrane antigen. These studies demonstrate that hrIL-2, as with antigens and phytomitogens, may serve as the first signal of T cell proliferation.

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