In vitro biosynthesis of prostaglandin E2 by kidney medulla of essential fatty acid deficient rats
- 1 September 1976
- Vol. 11 (9) , 693-696
- https://doi.org/10.1007/bf02532889
Abstract
Weanling rats were fed either a semi-synthetic diet with no fat, with 28% by wt partially hydrogenated fish oil, or with 28% by wt arachis oil (control diet) for 6 or 71/2 months. The in vitro conversion of arachidonic acid to prostaglandin E2 by homogenates of the rat kidney medulla was measured by gaschromatography with electron capture detection. The kidney medulla of essential fatty acid deficient animals showed increased activity for the in vitro conversion of exogenous arachidonic acid to prostaglandin E2 when compared to the controls. The change of the enzymatic activity in the essential fatty acid deficient animals was reversible, as shown by refeeding. Inhibition of the prostaglandin synthetase was found at exogenous substrate concentrations higher than 50–100 μM.This publication has 18 references indexed in Scilit:
- Enzymatic properties of prostaglandin synthetase from bovine seminal vesiclesBiochimie, 1975
- Some characteristics of the prostaglandin synthesizing system in rabbit kidney microsomesBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1975
- Enrichment of rat tissue lipids with fatty acids that are prostaglandin precursorsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1975
- Prostaglandin Synthesis in Membrane Fractions from the Rabbit Renal MedullaActa Physiologica Scandinavica, 1975
- Isolation and properties of intermediates in prostaglandin biosynthesisBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1973
- Studies on the synthesis of prostaglandins in the vesicular glands of essential fatty acid-deficient and hypophysectomized ratsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1973
- In vitro release of prostaglandins from the renal medullaEuropean Journal of Pharmacology, 1972
- Mechanism of prostaglandin biosynthesis. I. Characterization and assay of bovine prostaglandin synthetaseBiochemistry, 1971
- Inhibition of prostaglandin synthesis by oleic, linoieic and linoienic acidsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1968
- A new method for the preparation of diazomethaneRecueil des Travaux Chimiques des Pays-Bas, 1954