Rapid characterization of Mycobacterium fortuitum-chelonei complex by restriction fragment length polymorphism of ribosomal RNA genes
- 15 January 1991
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 77 (2-3) , 205-208
- https://doi.org/10.1016/0378-1097(91)90552-l
Abstract
Using labelled, γ-32P rRNA of mycobacteria as a probe restriction fragment length polymorphism (RFLP) of rRNA genes of strains belonging to the Mycobacterium fortuitum-chelonei complex was analysed. Each DNA sample was cleaved with EcoRI restriction endonuclease, the fragments were separated by agarose gel electrophoresis and transferred to nitrocellulose membrane. Fragments of DNA containing rRNA genes were identified by hybridization with γ-32P-labelled rRNA. Patterns were found to be species specific and both the species were distinguishable from each other. Results indicate that this approach can be used for rapid genomic characterization of the Mycobacterium fortuitum-chelonei complex.Keywords
This publication has 0 references indexed in Scilit: