Effects of 2-deoxy-D-glucose and quinidine on the fruiting body formation in Coprinus macrorhizus.

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Abstract
The addition of 2-deoxy-D-glucose and quinidine to culture media of strain Fisc of C. macrorhizus was effective in inhibiting fruiting body formation and in reducing the intracellular level of cAMP, adenylate cyclase and phosphodiesterase activities. 2-Deoxy-D-glucose taken into mycelial cells was phosphorylated and accumulated as 2-deoxy-D-glucose-monophosphate and 2-deoxy-D-glucose diphosphate. This phosphorylation appeared to be reversible. Quinidine was taken up by mycelial cells without metabolism. 2-Deoxy-D-glucose, 2-deoxy-D-glucosemonophosphate and quinidine inhibited adenylate cyclase activity but not phosphodiesterase activity measured in vitro. The Ki values of 2-deoxy-D-glucose and quinidine for adenylate cyclase activity were 1.8 and 0.08 mM, respectively.A possible relationship between the low adenylate cyclase activity and inhibition of fruiting body formation was indicated.

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