Effects of Dehydration-freezing-thawing on the Preservation and Homotransplantability of Renal Tissue

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Abstract
Experiments on kidney transplantation were carried out on white rats. Transplantation consisted of the insertion of a slice of kidney into a subcutaneous pocket in the abdominal wall. A total of 160 autotransplants and 300 homotransplants were made. It was found that the host reaction against the homograft consisted of marked lymphocytic infiltration and intense organization. Freeze dehydration at 0 C. of the transplants did not influence this reaction, while at temperatures of -90 and -190[degree] C. a marked inhibition of the lymphocytic infiltration, a marked decrease in the host connective tissue reaction, and an increase in the proliferative capacity of the vascular endothelium were noted. The collecting tubules were least sensitive, and convoluted tubules most sensitive to injury by freezing. The best results were observed when whole kidney was immersed in glycerin, frozen at -190[degree] C, and thawed by exposure at 100[degree]C.until the glycerin liquefied.