Screening Method for Detection of Drugs of Abuse in Human Urine
- 1 September 1971
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 17 (9) , 875-881
- https://doi.org/10.1093/clinchem/17.9.875
Abstract
A simple, convenient method is described for the detection in human urine of three major classes of drugs of abuse: amphetamines, barbiturates, and alkaloids. The drugs are adsorbed from urine by a column of non-ionic resin (Amberlite XAD-2), eluted with methanol, and chromatographed on a thin-layer silica gel plate. The drugs are made visible and identified by a series of procedures involving exposure to ultraviolet radiation, heat treatment, sequential spraying with group-specific reagents, and measurement of Rf values. Detection limits for 5-ml urine specimens are: 0.4 µg per ml for amphetamines and barbiturates, and 0.8 g per ml for alkaloids. The test requires 6 h to perform. One technologist can process 50 specimens per day.Keywords
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