The Effect of Carbon Dioxide Concentratioin, light Intensity andIsonicotinylHydrazide on the Photosynthetic Production of Glycollic Acid byChlorella

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Abstract
Glycollic acid production by Chlorella was measured by colorimetric determination of the acid excreted into the medium. It was found that glycollic acid production showed a maximum at a low concentration of carbon dioxide but tended toward zero as the rate of photosynthesis approached carbon dioxide saturation. Glycollic acid production became measurable at light intensities approaching that required to saturate photosynthesis and increased steadily with further increase in intensity. Treatment with isonicotinyl hydrazide resulted in an approximately threefold stimulation of glycollic acid concentration over the range of conditions used. It is suggested that the precursor of glycollic acid is ribulose diphosphate, and that isonicotinyl hydrazide acts by inhibiting the further metabolism of glycollic acid.

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