The Determination of Optimal Inoculum Quality for Submerse Fermentation Process
- 1 January 1992
- journal article
- Published by Institute of Organic Chemistry & Biochemistry in Collection of Czechoslovak Chemical Communications
- Vol. 57 (2) , 303-308
- https://doi.org/10.1135/cccc19920303
Abstract
The quantity and quality of inoculation material play a crucial role in bioprocess results. Instead of inexact decrease of pH value and subjective evaluation of the inoculum maturation, we proposed for the Streptomyces noursei cultivation to determine the rheological behaviour of the broth reflecting the growth of the culture and his physiological activity. For the rotational system cylinder-cylinder we found the optimal level of shear stress 80-120 mPa at shear rate 698 s-1 for the transfer of inoculum to production stage and achievement of maximal process productivity.Keywords
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