Studies in the biochemistry of micro-organisms. 112. Anthraquinone pigments of strains of Cladosporium fulvum Cooke

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Abstract
Three strains of Cladosporium fulvum, a wild strain and 2 mutants, were grown on Czapek-Dox medium supplemented by Marmite, and the mycelial pigments were examined. The pigment produced by 1 mutant was frangula-emodin; no pigment was obtained from the other mutant examined. The wild strain produced a new trihydroxymethylanthraquinone named cladofulvin, which yielded a triacetate and di- and tri-O-methyl derivatives. Distillation with Zn dust yielded 2-methylanthracene; oxidation of the dimethyl derivative of cladofulvin gave hemipinic acid. These facts, together with ultraviolet- and infrared-absorption spectra, indicated that cladofulvin has the structure l,2,8-trihydroxy-7-methylanthraquinone.

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