Histochemical and Tissue Culture Studies of Dystrophic and Experimentally Denervated Animal Muscle

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Abstract
Tissue cultures were established from the muscle explants of chicken and mice with hereditary muscular dystrophy as well as from normal muscles and muscles experimentally denervated at birth. Growth patterns and differentiation of fibers with special reference to histochemical maturation were examined in both normal and diseased muscles. The rate of growth of cultures of both dystrophic and denervated muscles of chicken and mice was accelerated as compared to cultures of normal muscle, and seemed to mature at a faster rate. Furthermore, histochemistry of muscle biopsies of young dystrophic muscles and muscles denervated at birth revealed striking morphological similarities, especially with regard to the presence of numerous small muscle fibers with central nuclei having the characteristics of regenerating fibers. It is not known at present whether in both young dystrophic and young denervated muscles the increased regeneration of muscle is due to the same or different factors. However, our results suggest that neural mechanisms in chicken and mouse hereditary muscular dystrophies should be taken into account.

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