Preparation of a Specific Antiserum against Elastin of the Human Aorta
- 1 January 1986
- journal article
- research article
- Published by S. Karger AG in Cells Tissues Organs
- Vol. 126 (1) , 68-72
- https://doi.org/10.1159/000146189
Abstract
Elastin was isolated from the human aorta by three different extraction methods. Immunization was carried out in sheep. The presence of antibody against each elastin preparation in the sheep sera was confirmed by immunofluorescence. However, antiserum against elastin isolated by collagenase/guanidine dithioerythritol showed the most specific reactions in the cryostat sections. No cross-reactivity to type I, III and IV collagen, fibronectin, laminin and proteoglycan BM 1 was observed.Keywords
This publication has 1 reference indexed in Scilit:
- Enzyme-linked immunosorbent microassay for quantification of specific antibodies to collagen type I, II, IIIJournal of Immunological Methods, 1979