Determination of fluorescent whitening agents in river waters with high performance liquid chromatography.

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Abstract
A simultaneous method for the determination of fluorescent Whitening agents (FWA), such as disodium 4,4''-bis[(4-anilino-6-morpholino-1,3,5-triazin-2-yl) amino] stilbene-2,2''-disulfonate (FB 71, trans isomer), disodium 4,4''-bis [(4-toluidino-6-morpholino-1,3,5-triazin-2-yl) amino] stilbene-2,2''-disulfonate (FB 225, trans isomer and cis isomer) and disodium 4,4''-bis (2-sulfo-styryl) biphenyl (FB 351, trans isomer and cis isomer), in river waters with high performance liquid chromatography (HPLC) was developed. FWA in river waters were adsorbed on Sep-pak C18 cartridge and eluted with methanol. The eluate was evaporated to dryness, and the residue was dissolved in acetonitrile-water (3:7). The solution was filtrated and analysed by HPLC under the following conditions: column, Inertsil ODS 5 (4.6 mm i.d. .times. 150 mm); mobile phase, acetonitrile-0.01 M monobasic sodium phosphate (35:65); flow rate, 1.0 ml/min; detections, ultraviolet detector (detection wavelength, 270 nm), fluorescent detector (excitation 345 nm and emission 430 nm). The average recoveries of FWA from river waters at 2-15.6 .mu.g/l were 90-102%. This method was applicable to determine the concentration of 0.01 .mu.g/l for FB 351, 0.05 .mu.g/l for FB 71 (trans), 0.6 .mu.g/l for FB 71 (cis), 0.1 .mu.g/l for FB 225 (trans) and 1.1 .mu.g/l for FB 225 (cis), respectively, using 100 ml samples. On the basis of the present method applied to samples from 6 different rivers located at Kanagawa prefecture, FB 351 (trans), FB 71 (trans, cis) and FB 225 (trans, cis) were determined.

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